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KMID : 0613820080180010075
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Journal of Life Science
2008 Volume.18 No. 1 p.75 ~ p.83
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Effects of Inhibitors on the Function and Activity of Topoisomerase, and Gene Expression in HL-60 Human Leukemia Cells
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Jeong In-Cheol
Cho Moo-Youn
Park Jang-Su
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Abstract
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This studies were designed to elucidate whether inhibitors of topoisomerase regulate function and activity of topoisomerase, and gene expression in HL-60 human leukemia cells. HL-60 cells were treated with 10-hydroxycamptothecin or doxorubicin, total RNA was isolated, and expressed genes were investigated with human oligonucleotide microarray containing 10K gene, respectively. Expression profiles of the human leukemia HL-60 cells treated with 10-hydroxycamptothecin (10-CIT) or doxorubicin associated with signal transduction,. cell adhesion, cell cycle, cell growth, cell proliferation, cell differentiation, transcription and immune response, especially genes related with transcription and cell growth. In HL-60 cells treated with 10-CPT, the expression of topoisomerase III, III and I gene from oligo chip microarray analysis were increased over, but the expression of topoisomerase II and II gene were decreased over. In contrast, the expression of topoisomerase II and II gene were increased over in HL-60 cells treated with doxorubicin, whereas the expression of topoisomerase III and III mRNA remained no significant change. These results suggest that these data may be useful for novel therapeutic markers.
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KEYWORD
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Topoisomerase, 10-hydroxycamptothecin (10-CPT), doxorubicin, expression profile, oligonucleotide microarray
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